Oxidase test: Difference between revisions
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==Background== |
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* Identifies '''cytochrome C oxidase''' by testing for the ability to oxidize tetramethyl-p-phenylenediamine to indophenols, which are purple |
* Identifies '''cytochrome C oxidase''' by testing for the ability to oxidize tetramethyl-p-phenylenediamine to indophenols, which are purple |
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* The test is positive when it turns '''purple''' within 10 seconds |
* The test is positive when it turns '''purple''' within 10 seconds |
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** Must be done from plain 5% SBA |
** Must be done from plain 5% SBA |
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==Interpretation== |
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* All oxidase-positive bacteria are aerobic |
* All oxidase-positive bacteria are aerobic |
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** '''P''': [[Pseudomonas species]] |
** '''P''': [[Pseudomonas species]] |
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** '''H''': [[Helicobacter species]] and [[Haemophilus species]] |
** '''H''': [[Helicobacter species]] and [[Haemophilus species]] |
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** [[Aeromonas species]] |
** [[Aeromonas species]] |
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===Oxidase-Positive Organisms=== |
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{{#ask: [[Oxidase::positive]] |?Stain |?Cellular shape |?Oxidase }} |
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===Oxidase-negative Organisms=== |
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{{#ask: [[Oxidase::negative]] |?Stain |?Cellular shape |?Oxidase }} |
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== Further Reading == |
== Further Reading == |
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Revision as of 19:54, 5 September 2021
Background
- Identifies cytochrome C oxidase by testing for the ability to oxidize tetramethyl-p-phenylenediamine to indophenols, which are purple
- The test is positive when it turns purple within 10 seconds
- Must be done from plain 5% SBA
Interpretation
- All oxidase-positive bacteria are aerobic
Oxidase-Positive Organisms
| Stain | Shape | Oxidase | |
|---|---|---|---|
| Achromobacter | Gram-negative | bacillus | positive |
| Aeromonas | Gram-negative | bacillus | positive |
| Alcaligenes faecalis | Gram-negative | bacillus | positive |
| Brucella melitensis | Gram-negative | coccobacillus | positive |
| Burkholderia pseudomallei | Gram-negative | bacillus | positive |
| Campylobacter | Gram-negative | bacillus | positive |
| Francisella tularensis | Gram-negative | coccobacillus | positive negative |
| Helicobacter pylori | Gram-negative | bacillus | positive |
| Herbaspirillum | Gram-negative | bacillus | positive |
| Micrococcus | Gram-positive | coccus | positive |
| Moraxella catarrhalis | Gram-negative | diplococcus | positive |
| Neisseria | Gram-negative | diplococcus | positive |
| Ochrobactrum anthropi | Gram-negative | bacillus | positive |
| Pasteurella | Gram-negative | bacillus | positive |
| Pseudomonas aeruginosa | Gram-negative | bacillus | positive |
| Vibrio | Gram-negative | bacillus | positive |
| Vibrio cholerae | Gram-negative | bacillus | positive |
| Vibrionaceae | Gram-negative | bacillus | positive |
Oxidase-negative Organisms
| Stain | Shape | Oxidase | |
|---|---|---|---|
| Acinetobacter ursingii | Gram-negative | coccobacillus | negative |
| Cronobacter sakazakii | Gram-negative | bacillus | negative |
| Erysipelothrix rhusiopathiae | Gram-positive | bacillus | negative |
| Francisella tularensis | Gram-negative | coccobacillus | positive negative |
| Listeria monocytogenes | Gram-variable Gram-positive | bacillus pleomorphic | negative |
| Proteus | Gram-negative | bacillus | negative |
| Raoultella | Gram-negative | bacillus | negative |
| Raoultella ornithinolytica | Gram-negative | bacillus | negative |
| Stenotrophomonas maltophilia | Gram-negative | bacillus | negative |
Further Reading
- Microbe Online. Oxidase Test: Principle, Procedure and Oxidase-Positive Organisms.
