Urease test
From IDWiki
- Tests for the ability of the bacteria to metabolize urea by using phenol red to detect a pH change from lysis of urea into ammonia and carbon dioxide
- Often found in bacteria that cause urinary tract infections
- Procedure
- Into a tube with urea medium, stab an inoculum of the isolate into the medium then streak the slant
- Incubate with a loose lid at 35ºC for 24 hours
- Positive if the orange-yellow color turns bright pink
- Common urease-positive organisms include
