Penicillin-binding protein

• A group of enzymes including transpeptidases, carboxypeptidases, and endopeptidases that play a role in the formation and maintenance of the cell wall structure

	Class A							Class B										Class C
	A1	A2	A3	A4	A5	A6	A7	B1	B2	B3	B4	B5	B5	B6	B-like-I	B-like-II	B-like-III	Type-4	Type-5	Type-7	Type-AmpH
Gram-Negative Bacteria
Escherichia coli	PBP1a ponA	PBP1b ponB				PBP1c pbpC	MGT mgt		PHP2 pbpA	PHP3 ftsI								PBP4 dacB	PBP5, PHP6, PHP6b dacA, dacC, dacD	PBP7 pbpG	PBP4b, AmpH yefw, ampH
Neisseria gonorrhoeae	PBP1 ponA									PBP2 ftsI								PBP3 pbp3		PBP4 pbp4
Gram-Positive Bacteria
Staphylococcus aureus			PBP2 pbp2				MGT mgt	PBP2a mecA			PBP1 pbpA	PBP3 pbp3							PBP4 pbp4
Listeria monocytogenes			PBP1	PBP4				PBP			PBP2	PBP3							PBP5
Enterococcus faecalis			PBP1a	PBP2a	PBP1b			PBP4			PBP2	PBP2b							DacF
Streptococcus pneumoniae			PBP1a pbpA	PBP2a pbp2&	PBP1b pbp1b						PBP2x pvpX	PBP2b pbp2b							PBP3 pbp3
Other Bacteria
Mycobacterium tuberculosis				PBP1 ponA1			PBP1A (r) ponA2		PBPA pbpA		PBP2 pbpB					PBP-lipo Rv2864c		PBP4 Rv3627	PBP5 dacB1	PBP7 dacB2	PBP

PBP Mutations

• Escherichia coli: PBP3 insert (YRIN/YRIK)
• Klebsiella pneumoniae: PBP1, PBP2, and PBP3
• Pseudomonas aeruginosa: PBP1 and PBP3
• Acinetobacter baumannii: PHP3-H370Y, PBP3-Q488K and Y528H

Antibiotic Targets

• MSSA
  • Cefazolin: primarily PBP2, but all except PBP4
  • Ertapenem: PBP1
  • Cefaclor: PBP3
  • Cefoxitin: PBP4