Catalase test: Difference between revisions
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*Used to differentiate a number of bacteria: |
*Used to differentiate a number of bacteria: |
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**[[Staphylococcus species]] (positive) from [[Streptococcus species]] and [[Enterococcus species]] (negative) |
**[[Staphylococcus species]] (positive) from [[Streptococcus species]] and [[Enterococcus species]] (negative) |
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** |
**[[Bacillus species]] (positive) from aerotolerant strains of [[Clostridium species]] (negative) |
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**Members of the [[Enterobacteriaceae]] family (positive) from other Gram-negative bacilli |
**Members of the [[Enterobacteriaceae]] family (positive) from other Gram-negative bacilli |
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*Detects the presence of catalase, which catalyzes the conversion of hydrogen peroxide into water and oxygen |
*Detects the presence of catalase, which catalyzes the conversion of hydrogen peroxide into water and oxygen |
Revision as of 01:09, 28 July 2020
- Used to differentiate a number of bacteria:
- Staphylococcus species (positive) from Streptococcus species and Enterococcus species (negative)
- Bacillus species (positive) from aerotolerant strains of Clostridium species (negative)
- Members of the Enterobacteriaceae family (positive) from other Gram-negative bacilli
- Detects the presence of catalase, which catalyzes the conversion of hydrogen peroxide into water and oxygen
- Mix a small sample of bacteria with 3% hydrogen peroxide
- Positive if bubbles appears within 10 seconds
- Negative if no or only a few bubbles