Gram stain: Difference between revisions
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== Procedure == |
== Procedure == |
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| − | * Fix to slide with heat |
+ | * Fix to slide with heat or methanol |
| − | * |
+ | * Add crystal violet, and wait 60 seconds |
* Wash with water then flood with iodine, and wait 60 seconds |
* Wash with water then flood with iodine, and wait 60 seconds |
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| − | * Wash with water then decolorize with 95% |
+ | * Wash with water then decolorize with 95% ethanol or acetone |
* Counter-stain with safrinin, wait 30 seconds then wash off with water |
* Counter-stain with safrinin, wait 30 seconds then wash off with water |
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Latest revision as of 19:50, 29 April 2020
Procedure
- Fix to slide with heat or methanol
- Add crystal violet, and wait 60 seconds
- Wash with water then flood with iodine, and wait 60 seconds
- Wash with water then decolorize with 95% ethanol or acetone
- Counter-stain with safrinin, wait 30 seconds then wash off with water
Interpretation
- Gram-positive = blue
- Gram-negative = red
- Peptidoglycan layer (cell wall) is thick with extensive cross-linking in gram-positive
