Gram stain: Difference between revisions

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* Peptidoglycan layer (cell wall) is thick with extensive cross-linking in gram-positive
* Peptidoglycan layer (cell wall) is thick with extensive cross-linking in gram-positive


[[Category:Microbiology]]
[[Category:Diagnostics]]

Revision as of 11:15, 16 August 2019

Procedure

  • Fix to slide with heat
  • Crystal violet, and wait 60 seconds
  • Wash with water then flood with iodine, and wait 60 seconds
  • Wash with water then decolorize with 95% alcohol
  • Counter-stain with safrinin, wait 30 seconds then wash off with water

Interpretation

  • Gram-positive = blue
  • Gram-negative = red
  • Peptidoglycan layer (cell wall) is thick with extensive cross-linking in gram-positive