Coagulase test: Difference between revisions
From IDWiki
No edit summary |
No edit summary |
||
| Line 10: | Line 10: | ||
**Mix a heavy inoculum of bacteria into a tube filled with rabbit plasma |
**Mix a heavy inoculum of bacteria into a tube filled with rabbit plasma |
||
**Positive if clot is formed within 4 hours when incubated at 37ºC |
**Positive if clot is formed within 4 hours when incubated at 37ºC |
||
**A few species of [[Coagulase-negative staphylococci]] may be positive: [[Staphylococcus intermedius]], [[Staphylococcus saccharolyticus]], and [[Staphylococcus schleiferi]] |
**A few species of [[Coagulase-negative staphylococci]] may be positive: [[Staphylococcus intermedius]], [[Staphylococcus hyicus]], [[Staphylococcus saccharolyticus]], and [[Staphylococcus schleiferi]] |
||
[[Category:Diagnostics]] |
[[Category:Diagnostics]] |
||
Revision as of 22:36, 23 August 2020
- Used to differentiate Staphylococcus aureus from less pathogenic coagulase-negative staphylococci
- Tests for the ability of the organism to convert fibrinogen into fibrin (that is, to form a clot)
- Slide coagulase
- Checks for agglutination after a drop of plasma is combined with a suspension of the bacteria on a slide
- Positive if clumping is apparent within 10 seconds or less, and no clumping is observed in saline or water
- A few species of coagulase-negative staphylococci may be positive due to membrane-bound clumping factor
- Staphylococcus lugdenensis, Staphylococcus schleiferi subsp. schleiferi, Staphylococcus sciuri subsp. carnaticus, and Staphylococcus sciuri subsp. rodentium
- Staphylococcus aureus is negative in 15% of tests, especially MRSA, so negative results need to be confirmed with tube coagulase
- Tube coagulase
- Mix a heavy inoculum of bacteria into a tube filled with rabbit plasma
- Positive if clot is formed within 4 hours when incubated at 37ºC
- A few species of Coagulase-negative staphylococci may be positive: Staphylococcus intermedius, Staphylococcus hyicus, Staphylococcus saccharolyticus, and Staphylococcus schleiferi
References
- ^ Takashi Sasaki, Sae Tsubakishita, Yoshikazu Tanaka, Arihito Sakusabe, Masayuki Ohtsuka, Shintaro Hirotaki, Tetsuji Kawakami, Tsuneo Fukata, Keiichi Hiramatsu. Multiplex-PCR Method for Species Identification of Coagulase-Positive Staphylococci. Journal of Clinical Microbiology. 2010;48(3):765-769. doi:10.1128/jcm.01232-09.
