Coagulase test: Difference between revisions

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**Checks for agglutination after a drop of plasma is combined with a suspension of the bacteria on a slide
**Checks for agglutination after a drop of plasma is combined with a suspension of the bacteria on a slide
**Positive if clumping is apparent within 10 seconds or less, and no clumping is observed in saline or water
**Positive if clumping is apparent within 10 seconds or less, and no clumping is observed in saline or water
**A few species of [[coagulase-negative staphylococci]] may be positive, including [[Staphylococcus lugdenensis]] and [[Staphylococcus intermedius]]
**A few species of [[coagulase-negative staphylococci]] may be positive due to membrane-bound clumping factor, including [[Staphylococcus lugdenensis]] and [[Staphylococcus intermedius]]
**[[Staphylococcus aureus]] is negative in 15% of tests, especially MRSA, so negative results need to be confirmed with tube coagulase
**[[Staphylococcus aureus]] is negative in 15% of tests, especially MRSA, so negative results need to be confirmed with tube coagulase
*'''Tube coagulase'''
*'''Tube coagulase'''

Revision as of 22:20, 23 August 2020

References

  1. ^  Takashi Sasaki, Sae Tsubakishita, Yoshikazu Tanaka, Arihito Sakusabe, Masayuki Ohtsuka, Shintaro Hirotaki, Tetsuji Kawakami, Tsuneo Fukata, Keiichi Hiramatsu. Multiplex-PCR Method for Species Identification of Coagulase-Positive Staphylococci. Journal of Clinical Microbiology. 2010;48(3):765-769. doi:10.1128/jcm.01232-09.