Catalase test: Difference between revisions
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**[[Staphylococcus species]] (positive) from [[Streptococcus species]] and [[Enterococcus species]] (negative) |
**[[Staphylococcus species]] (positive) from [[Streptococcus species]] and [[Enterococcus species]] (negative) |
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**[[Bacillus species]] (positive) from aerotolerant strains of [[Clostridium species]] (negative) |
**[[Bacillus species]] (positive) from aerotolerant strains of [[Clostridium species]] (negative) |
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**Members of the [[Enterobacteriaceae]] family (positive) from other Gram-negative bacilli |
**Members of the [[Enterobacteriaceae]] family (positive) from other [[Gram-negative bacilli]] |
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*Detects the presence of catalase, which catalyzes the conversion of hydrogen peroxide into water and oxygen |
*Detects the presence of catalase, which catalyzes the conversion of hydrogen peroxide into water and oxygen |
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*Mix a small sample of bacteria with 3% hydrogen peroxide |
*Mix a small sample of bacteria with 3% hydrogen peroxide |
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**Positive if bubbles appears within 10 seconds |
**Positive if bubbles appears within 10 seconds |
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**Negative if no or only a few bubbles |
**Negative if no or only a few bubbles |
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*Bacteria that are catalase positive: [[staphylococci]], [[Micrococcus]], [[Listeria]], [[Corynebacterium diphtheriae]], [[Burkholderia cepacia]], [[Nocardia]], the family [[Enterobacteriaceae]] ([[Citrobacter]], [[E. coli]], [[Enterobacter]], [[Klebsiella]], [[Shigella]], [[Yersinia]], [[Proteus]], [[Salmonella]], [[Serratia]]), [[Pseudomonas]], [[Mycobacterium tuberculosis]], [[Aspergillus]], [[Cryptococcus]], and [[Rhodococcus equi]] |
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*Bacteria that are catalase negative: [[Streptococcus]], [[Enterococcus]], [[Pediococcus]], [[Leuconostoc]], [[Aerococcus]], [[Gemella]] |
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[[Category:Diagnostics]] |
[[Category:Diagnostics]] |
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Revision as of 12:26, 31 August 2021
- Used to differentiate a number of bacteria:
- Staphylococcus species (positive) from Streptococcus species and Enterococcus species (negative)
- Bacillus species (positive) from aerotolerant strains of Clostridium species (negative)
- Members of the Enterobacteriaceae family (positive) from other Gram-negative bacilli
- Detects the presence of catalase, which catalyzes the conversion of hydrogen peroxide into water and oxygen
- Mix a small sample of bacteria with 3% hydrogen peroxide
- Positive if bubbles appears within 10 seconds
- Negative if no or only a few bubbles
- Bacteria that are catalase positive: staphylococci, Micrococcus, Listeria, Corynebacterium diphtheriae, Burkholderia cepacia, Nocardia, the family Enterobacteriaceae (Citrobacter, E. coli, Enterobacter, Klebsiella, Shigella, Yersinia, Proteus, Salmonella, Serratia), Pseudomonas, Mycobacterium tuberculosis, Aspergillus, Cryptococcus, and Rhodococcus equi
- Bacteria that are catalase negative: Streptococcus, Enterococcus, Pediococcus, Leuconostoc, Aerococcus, Gemella
