Optochin sensitivity test: Difference between revisions
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* A test applied to alpha-hemolytic streptococci in order to presumptively identify [[Streptococcus |
* A test applied to alpha-hemolytic streptococci in order to presumptively identify '''[[Streptococcus pneumoniae]]''', which is susceptible to optochin (although not all strains are susceptible) |
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* Apply an optochin or P disc to an SBA plate with freshly-streaked inoculum, then incubate at 35-37°C with 5% CO<sub>2</sub> for 18 to 24 hours |
* Apply an optochin or P disc to an SBA plate with freshly-streaked inoculum, then incubate at 35-37°C with 5% CO<sub>2</sub> for 18 to 24 hours |
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* Measure the zone of inhibition |
* Measure the zone of inhibition |
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Latest revision as of 12:52, 29 October 2019
- A test applied to alpha-hemolytic streptococci in order to presumptively identify Streptococcus pneumoniae, which is susceptible to optochin (although not all strains are susceptible)
- Apply an optochin or P disc to an SBA plate with freshly-streaked inoculum, then incubate at 35-37°C with 5% CO2 for 18 to 24 hours
- Measure the zone of inhibition
- Using a standard 6 mm disc, a zone of 14 mm or more indicates susceptibility and suggests Streptococcus pneumoniae
- Any zone of inhibition is uninterpretable, and should be followed by a bile solubility test
