Gram stain: Difference between revisions
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Revision as of 01:16, 15 August 2019
Gram stain
Procedure
- Fix to slide with heat
- Crystal violet, and wait 60 seconds
- Wash with water then flood with iodine, and wait 60 seconds
- Wash with water then decolorize with 95% alcohol
- Counter-stain with safrinin, wait 30 seconds then wash off with water
Interpretation
- Gram-positive = blue
- Gram-negative = red
- Peptidoglycan layer (cell wall) is thick with extensive cross-linking in gram-positive
