Gram stain: Difference between revisions
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= Gram stain = |
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== Procedure == |
== Procedure == |
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* Fix to slide with heat |
* Fix to slide with heat or methanol |
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* Add crystal violet, and wait 60 seconds |
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* Wash with water then flood with iodine, and wait 60 seconds |
* Wash with water then flood with iodine, and wait 60 seconds |
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* Wash with water then decolorize with 95% |
* Wash with water then decolorize with 95% ethanol or acetone |
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* Counter-stain with safrinin, wait 30 seconds then wash off with water |
* Counter-stain with safrinin, wait 30 seconds then wash off with water |
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* Gram-negative = red |
* Gram-negative = red |
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* Peptidoglycan layer (cell wall) is thick with extensive cross-linking in gram-positive |
* Peptidoglycan layer (cell wall) is thick with extensive cross-linking in gram-positive |
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[[Category:Diagnostics]] |
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Latest revision as of 00:50, 30 April 2020
Procedure
- Fix to slide with heat or methanol
- Add crystal violet, and wait 60 seconds
- Wash with water then flood with iodine, and wait 60 seconds
- Wash with water then decolorize with 95% ethanol or acetone
- Counter-stain with safrinin, wait 30 seconds then wash off with water
Interpretation
- Gram-positive = blue
- Gram-negative = red
- Peptidoglycan layer (cell wall) is thick with extensive cross-linking in gram-positive
